inquiry@rdetect.com 【Introduction】
Norovirus (NoV), also known as norovirus, belongs to the calciviruses family and is mainly transmitted through contaminated water, food, contact or aerosols formed by pollutants. It has been recognized by European and American countries as the primary pathogen causing viral diarrhea and gastroenteritis in adults, and the second pathogen causing viral diarrhea in children, second only to rotavirus. Norovirus plays an important role in the outbreak of non-bacterial gastroenteritis, which is associated with 30% to 50% of the aseptic gastroenteritis, and 90% of the viral gastroenteritis caused by food is caused by norovirus “. Norovirus is mainly divided into 5 genomes (GI, GII, GIII, GIV and GV), mainly infecting people are GI, GII and GIV, among which norovirus of GII genome is the most common virus strain worldwide 5.6. Clinical or laboratory diagnosis of norovirus infection mainly includes electron microscopy, molecular biology and immunological detection methods. This kit AIDS in the diagnosis of acute viral gastroenteritis, for the rapid diagnosis of the disease to determine the pathogen in time, avoid hospitals or hospitals.
【Sample Collection&Storage】
Collect stool specimens as soon as possible after symptom onset. If not detected immediately, they can be stored at 2-8 ºC for 2 days. Long-term storage needs to be frozen at -20ºC, which can be stored for 3 years. Aseptic operation should be paid attention to in the collection and storage process of the preserved species, and they should be thawed thoroughly before use and returned to room temperature for testing. When repeated freezing and thawing samples are required, the amount of freezing and thawing shall not exceed 3 times.
【Operation】
Bring tests, specimens, buffer and/or controls to room temperature (15- 30°C) before use.
1. Add 0.5ml diluent in test tube.
2. Add proper amount of stool sample (about 50mg for solid and 50mL for liquid) into the test tube, so that the concentration is about 5-10%.
3. Mix well.
4. Take 2~3 drops of mixed samples (about 80pL) with an eyedropper and add them to the adding place at the lower end of the indicator arrow of the detection bar for 5~15 minutes for interpretation. The interpretation is invalid after 15 minutes.
